JNK Inhibition Modulates the Cytoskeleton, Hypoxia, and Neurogenesis on the Protein Level in Glioblastoma Cells and Astrocytes: An Immunofluorescence Study
Küçük Resim Yok
Tarih
2023
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Turkish Neurosurgical Society
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
AIM: To evaluate the effects of c-Jun N-terminal kinase (JNK) inhibition and signal blocking on hypoxia (hypoxia-inducible factor 1-alpha (HIF-1?)), differentiation and neurogenesis (bone morphogenetic protein (BMP4)), and the cytoskeleton (F-actin) in glioblastoma multiforme cells (GBMCs). MATERIAL and METHODS: We evaluated the differences between GBMCs and astrocytes in terms of the abovementioned parameters and assessed them with the aim of studying human GBMCs (U-87 MG) and astrocytes (SVG p12). The cells were exposed to different doses of the JNK inhibitor, SP600125, for 24, 48, and 72 hours. HIF-1?, BMP4, and F-actin expressions were evaluated using immunofluorescence image analysis. RESULTS: The half-maximal inhibitory concentration value for SP600125 was determined to be 10 ?M at 24 hours of exposure. After SP600125 administration, elevated levels of HIF-1? and BMP4 were detected in GBMCs and astrocytes. F-actin level only increased in GBMCs after SP600125 administration. CONCLUSION: JNKs are important for cell proliferation, differentiation, survival, and death; thus, research on JNKs has become important for the treatment of many human diseases, especially brain tumors, Parkinson’s disease, and Alzheimer’s disease. The results of this study involving immunofluorescence techniques should be investigated and supported by studies that involve comprehensive molecular techniques. © (2023), (Turkish Neurosurgical Society). All Rights Reserved.
Açıklama
Anahtar Kelimeler
BMP4, c-Jun N-terminal kinases, F-actin, Glioblastoma multiforme, HIF-1?, actin, pyrazolanthrone, astrocyte, cytoskeleton, fluorescent antibody technique, glioblastoma, human, hypoxia, metabolism, nervous system development, pathology, Actins, Astrocytes, Cytoskeleton, Fluorescent Antibody Technique, Glioblastoma, Humans, Hypoxia, Neurogenesis
Kaynak
Turkish Neurosurgery
WoS Q Değeri
Scopus Q Değeri
Q3
Cilt
33
Sayı
6
