Mikroalglerden nanopartikül üretimi ve karakterizasyonu
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Dosyalar
Tarih
2020
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Ege Üniversitesi, Fen Bilimleri Enstitüsü
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu tez çalışması kapsamında yeşil sentez yöntemi ile hücre içi ve hücre
dışı zengin metobolitler içeren Galdieria sulphuraria mikroalgi kullanılarak
titanyum dioksit ve gümüş nanopartikül sentezleme ve kozmetik alanında
kullanım potansiyeli araştırılmıştır.
İlk olarak ön optimizasyon çalışmaları doğrultusunda metal çözeltisi
konsantrasyonu ile hücre dışı metal: süpernatant ve hücre içi metal: biyokütle
oranı iki farklı parametre olarak optimize edilmiştir. Nanopartiküllerin
karakterizasyon analizi UV-Vis spektrofotometre, Zeta Sizer Nanorange ve
antibakteriyal aktivite testi ile gerçekleştirilmiştir. 3,4 mM Titanyum (IV)
bis(amonyum laktat) hidroksit metal çözeltisi derişimi ve 7,8 metal
çözeltisi/süpernatant (v/v) oranı ile optimum koşullarda nanopartiküller ile elde
edilmiştir. Kontrollü bir şekilde ölçek büyüterek titanyum dioksit nanopartiküller
sentezlenmesi için Foto-Biyojenik Nanopartikül Reaktör (FBNP) sistemi
kullanılmıştır. Bu reaktör sisteminde sentez sırasında nanopartikül boyut ve
stabilitesini etkileyen inkübasyon süresi, pH ve karıştırma hızı parametleri
optimize edilmiştir. Bu çalışma doğrultusunda pH 8,5‟te 285 rpm karıştırma hızı
ve 22 dakikalık inkübasyon süresi sonunda ortalama 120 nm çapında nanopartikül
üretilmiştir. Optimum koşullarda sentezlenen titanyum dioksit nanopartiküllerinin
karakterizasyonu işlemi gerçekleştirilerek kozmetik alanda kullanım potansiyelleri
in vitro hücre kültürü denemeleri ile incelenmiştir.
In this thesis, the aim is to investigate green synthesis of titanium dioxide and silver nanoparticle from Galdieria sulphuraria microalgae that containing rich intracellular and extracellular metabolites and potential use in cosmetics. At first step, in the line with preliminary optimization studies, extracellular metal: supernatant ratio and intracellular metal: biomass ratio with metal solution concentration as two different parameters were optimized. The characterization analysis of nanoparticles was carried out with UV-Vis spectrophotometer, Zetasizer Nano Range, and antibacterial activity test. Nanoparticles synthesized under optimum conditions with 3,4 mM Titanium (IV) bis (ammonium lactate) dihydroxide metal solution concentration and 7,8 metal solution/ supernatant (v/v) ratio. Photo-Biogenic Nanoparticle Reactor (FBNP) system was used to synthesize titanium dioxide nanoparticles by scaling up in a controlled manner. In this reactor system, incubation time, pH and mixing rate parameters that affect on nanoparticle size and stability were optimized. In reactor system, titanium nanoparticles with an average of 120 nm diameter were produced after 22 minutes incubation period at a pH of 8,5 at 285 rpm. Titanium dioxide nanoparticles produced under optimum conditions were characterized and the potential use in cosmetics was also investigated by in vitro cell culture assays.
In this thesis, the aim is to investigate green synthesis of titanium dioxide and silver nanoparticle from Galdieria sulphuraria microalgae that containing rich intracellular and extracellular metabolites and potential use in cosmetics. At first step, in the line with preliminary optimization studies, extracellular metal: supernatant ratio and intracellular metal: biomass ratio with metal solution concentration as two different parameters were optimized. The characterization analysis of nanoparticles was carried out with UV-Vis spectrophotometer, Zetasizer Nano Range, and antibacterial activity test. Nanoparticles synthesized under optimum conditions with 3,4 mM Titanium (IV) bis (ammonium lactate) dihydroxide metal solution concentration and 7,8 metal solution/ supernatant (v/v) ratio. Photo-Biogenic Nanoparticle Reactor (FBNP) system was used to synthesize titanium dioxide nanoparticles by scaling up in a controlled manner. In this reactor system, incubation time, pH and mixing rate parameters that affect on nanoparticle size and stability were optimized. In reactor system, titanium nanoparticles with an average of 120 nm diameter were produced after 22 minutes incubation period at a pH of 8,5 at 285 rpm. Titanium dioxide nanoparticles produced under optimum conditions were characterized and the potential use in cosmetics was also investigated by in vitro cell culture assays.
Açıklama
Anahtar Kelimeler
Metalik Nanopartikül, Galdieria Sulphuraria, Yeşil Sentez, Karakterizasyon, Biyoreaktör, Kozmetik, Metallic Nanoparticle, Galdieria Sulphuraria, Green Synthesis, Characterization, Bioreactor, Cosmetics
