Determination of extended spectrum beta-lactamase frequency of Klebsiella pneumoniae strains isolated from urinary tract infections and typing with isoelectric focusing method
Küçük Resim Yok
Tarih
2007
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Dergi ISSN
Cilt Başlığı
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Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu çalışmada, idrar yolu infeksiyonlarından izole edilen Klebsiella pneumoniae kökenlerinde genişlemiş spektrumlu beta-laktamaz (GSBL) varlığı araştırıldı ve tiplendirmeleri izoelektrik odaklama yöntemi ile yapıldı. Elli iki K. pneumoniae kökeninin antibiyotik duyarlılıkları disk difüzyon yöntemi ile belirlendi. On iki (% 23) kökende çift disk sinerji yöntemi ile GSBL varlığı saptandı. Bu kökenlerin seftazidim (CAZ) ve sefotaksim (CTX) minimum inhibitör konsantrasyon (MİK) değerleri E-test yöntemi kullanılarak, GSBL enzimlerinin izoelektrik noktaları ise poliakrilamid jel elektroforezi yöntemi ile belirlendi. Kökenlerin üçüncü jenerasyon sefalosporinler ve aztreonam duyarlılık paternleri dikkate alındığında, altı kökende en az bir antibiyotik ile GSBL öncü belirtisi saptandı. İzoelektrik odaklama yöntemi ile 12 kökenin 11’inde pI 7.6 olan bantlar gözlendi. Bu kökenlerin beşinde CAZ MİK’i 16 mg/mL ve üzerinde, CTX MİK’i 12 mg/mL ve üzerinde bulundu. Sonuç olarak, antibiyotik duyarlılık paternleri ve izoelektrik noktaları gözönüne alındığında bu beş kökende bulunan enzimlerin SHV-2 olabileceği düşünüldü.
Extended spectrum beta-lactamase (ESBL) presence was investigated in Klebsiella pneumoniae strains which were the ethiological agents of urinary tract infections and typed with isoelectric focusing (IEF) method. The antibiotic sensitivities of 52 K. pneumoniae strains were determined by disc diffusion method and ESBL were found in 12 strains (23 %) by double disc synergy test. Minimum inhibitory concentration (MIC) values of ESBL (+) K. pneumoniae strains for ceftazidime (CAZ) and cefotaxime (CTX) were investigated by E-test and isoelectric points of ESBL enzymes were determined by polyacrilamide gel electrophoresis. When the strains’ sensitivity patterns were investigated for 3rd generation cephalosporins and aztreonam, 6 strains had ESBL premise sign with at least one antibiotic. Band pattern which focused around pl 7.6 was determined in 11 of 12 strains by IEF method. MIC values were found 16 mg/mL and over for CAZ and 12 mg/mL and over for CTX in 5 of these strains. When pI’s and antibiotic susceptibility patterns were analysed, 5 of 12 isolates had a band and susceptibility patterns suggesting SHV-2.
Extended spectrum beta-lactamase (ESBL) presence was investigated in Klebsiella pneumoniae strains which were the ethiological agents of urinary tract infections and typed with isoelectric focusing (IEF) method. The antibiotic sensitivities of 52 K. pneumoniae strains were determined by disc diffusion method and ESBL were found in 12 strains (23 %) by double disc synergy test. Minimum inhibitory concentration (MIC) values of ESBL (+) K. pneumoniae strains for ceftazidime (CAZ) and cefotaxime (CTX) were investigated by E-test and isoelectric points of ESBL enzymes were determined by polyacrilamide gel electrophoresis. When the strains’ sensitivity patterns were investigated for 3rd generation cephalosporins and aztreonam, 6 strains had ESBL premise sign with at least one antibiotic. Band pattern which focused around pl 7.6 was determined in 11 of 12 strains by IEF method. MIC values were found 16 mg/mL and over for CAZ and 12 mg/mL and over for CTX in 5 of these strains. When pI’s and antibiotic susceptibility patterns were analysed, 5 of 12 isolates had a band and susceptibility patterns suggesting SHV-2.
Açıklama
Anahtar Kelimeler
Farmakoloji ve Eczacılık
Kaynak
Turkish Journal of Pharmaceutical Sciences
WoS Q Değeri
Scopus Q Değeri
Cilt
4
Sayı
3
