DNA biosensor for Microcystis spp. sequence detection by using methylene blue and ruthenium complex as electrochemical hybridization labels
Küçük Resim Yok
Tarih
2002
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Scientific Technical Research Council Turkey
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
An electrochemical biosensor for the voltammetric detection of DNA sequences related to the bloom-forming genera of cyanobacteria, Microcystis spp., is described. A specific DNA "probe" is designed based on sequence polymorphism within the 16S ribosomal DNA (rDNA) of the Microcystis spp. These single-stranded probes were immobilized onto carbon paste electrode (CPE) with the adsorption at a controlled potential. The probes were hybridized with the complementary "target" sequences at the electrode. The formed hybrids on the electrode surface were evaluated by differential pulse voltammetry (DPV) and cyclic voltammetry (CV) using methylene blue (MB) and tris (2,2' - bipyridine) ruthenium (H) chloride ([Ru(bpy)(3)](2+)) as the labels of hybridization. The response of the probe modified CPE to the exposure of the non-complementary oligonucleotide proves the specificity of the hybridization with the target. The two-bases mismatch could also be discriminated and specific detection of Microcystis spp. was achieved by using the difference between the voltammetric peaks of MB and [Ru(bpy)(3)](2+) obtained with the probe and hybrid-modified CPEs. The detection of Microcystis spp. target DNA from real tap water and river water samples was also achieved.
Açıklama
Anahtar Kelimeler
DNA biosensor, Microcystis spp., methylene blue, ruthenium bipyridine
Kaynak
Turkish Journal of Chemistry
WoS Q Değeri
Q3
Scopus Q Değeri
N/A
Cilt
26
Sayı
6
