Immobilization of phospholipase A(2) on porous glass and its application for lowering serum cholesterol concentration
Küçük Resim Yok
Tarih
2003
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Marcel Dekker Inc
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Phospholipase A(2) (PLA(2); EC 3.1.1.4) is a lipolytic enzyme that hydrolysis the ester bond in sn-2 position of phospholipids. In this work, the PLA(2) from hog pancreas was covalently coupled to porous glass. The properties of free and immobilized enzyme were also investigated and compared. The optimum pH and temperature were found as 8.5 and 50degreesC, respectively for both free and immobilized enzyme. The immobilized enzyme had good properties that potential for medical application is considerable. Its use in lowering plasma cholesterol concentrations in blood samples was also demonstrated.
Açıklama
Anahtar Kelimeler
phospholipase A(2), porous glass, immobilization, cholesterol
Kaynak
Artificial Cells Blood Substitutes and Immobilization Biotechnology
WoS Q Değeri
Q3
Scopus Q Değeri
Cilt
31
Sayı
4
