Preliminary investigations on suppression of meloidogyne incognita (Kofoid & White) chitwood (Nematoda: Heteroderidae) by antagonistic rhizobacteria
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Tarih
2006
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info:eu-repo/semantics/openAccess
Özet
Floresan Pseudomonad ırkları nematod ile bulaşık domates köklerinde bulunan hastalıklı Meloidogyne incognita (Kofoid & White, 1919) Chitwood, 1949 (Nematoda: Heteroderidae) 2. dönem larvalarından elde edilmiştir. In vivo’da 5 farklı ırk izole edilmiş, Kökur nematodlarına antagonist etkilerini test etmek amacıyla bu ırklar içinden patojen olmadığı tespit edilen Pseudomonas fluorescens Migula, 1895 (Proteobacteria: Pseudomonadaceae) Strain Pat1 seçilmiştir. Denemelerde hıyar (Cucumis sativus) cv Sardes test bitkisi olarak kullanılmıştır. Bakteri süspansiyonu (109 cfu/ml) üç farklı şekilde uygulanmıştır: 1) Tohum bakterizasyon; 2) Tohum bakterizasyon ve fide içirme ve 3) Fide içirme. Hıyar bitkilerinin fidelerini kök-ur nematodları ile bulaştırmak için, kök-ur nematodlarının yumurtaları bulaşık domates bitkilerinin köklerinden blender-elek yöntemiyle elde edilmiştir. Şaşırtma esnasında hıyar fideleri 20 000 yumurta/bitki ile bulaştırılmıştır. Bitkiler, kontrollü koşullara sahip olan klimatize edilmiş odalarda belirti gelişimi için 10–11 hafta gözlenmiştir. Belirti gelişimi, bitkilerin toprak üstü kısımları için 0–4 skalası, kökleri içinde 0–10 Zeck Kök-ur skalası kullanılarak değerlendirilmiştir. Tohum kaplama ile tohum kaplama ve fide daldırma, sadece kök-ur nematodları ile bulaşık bitkilerin bulunduğu pozitif kontrol ile karşılaştırıldığında, bitkilerin toprak üstü kısımlarındaki hastalık gelişiminin sırasıyla % 35 ve % 21, köklerdeki ur oluşumunun % 44 ve % 39 oranlarında düştüğü belirlenmiştir. Deneme, köklerdeki antagonist bakterilerin kolonizasyonu ve populasyon dinamiğini gözlemek amacıyla iki kez yinelenmiştir.
Fluorescent Pseudomonad strains have been obtained from diseased second juveniles from Meloidogyne incognita (Kofoid & White, 1919) Chitwood, 1949 (Nematoda: Heteroderidae) infected tomato roots. After the screening in vivo made by 5 different isolated strains, Strain Pat1 has been selected to test the antagonistic effects on the root-knot nematodes. Strain Pat1 has been identified as non plant pathogenic Pseudomonas fluorescens Migula, 1895 (Proteobacteria: Pseudomonadaceae) according to the test result. Cucumber (Cucumis sativus) cv Sardes has been used as test plant. Bacterial suspension (109 cfu/ml) was applied by three different ways: 1) Seed bacterization; 2) Seed bacterization plus seedling drenching; and 3) Seedling drenching. The eggs of rootknot nematodes have been obtained from diseased tomato roots by blender-sieve method for infection cucumber seedlings with root-knot nematodes. 20 000 eggs/plant have been given to cucumber seedlings during transplanting. The plants have been observed for symptom development for 10-11 weeks in climatized room under controlled conditions. Symptom development has been evaluated by using 0-4 scale for upper parts of plants, and 0-10 Zeck’s root-knot scale for the roots. When seed bacterization and seed bacterization plus seedling drenching applications compared with positive control; plants infected with root-knot nematodes alone, it was determined that disease symptoms on upper parts of the plants were reduced 35 % and 21 %; gal formation on the roots were reduced 44 % and 39 %,respectively. The experiment has been repeated twice to observe the colonization and population dynamic of antagonistic bacteria on the roots.
Fluorescent Pseudomonad strains have been obtained from diseased second juveniles from Meloidogyne incognita (Kofoid & White, 1919) Chitwood, 1949 (Nematoda: Heteroderidae) infected tomato roots. After the screening in vivo made by 5 different isolated strains, Strain Pat1 has been selected to test the antagonistic effects on the root-knot nematodes. Strain Pat1 has been identified as non plant pathogenic Pseudomonas fluorescens Migula, 1895 (Proteobacteria: Pseudomonadaceae) according to the test result. Cucumber (Cucumis sativus) cv Sardes has been used as test plant. Bacterial suspension (109 cfu/ml) was applied by three different ways: 1) Seed bacterization; 2) Seed bacterization plus seedling drenching; and 3) Seedling drenching. The eggs of rootknot nematodes have been obtained from diseased tomato roots by blender-sieve method for infection cucumber seedlings with root-knot nematodes. 20 000 eggs/plant have been given to cucumber seedlings during transplanting. The plants have been observed for symptom development for 10-11 weeks in climatized room under controlled conditions. Symptom development has been evaluated by using 0-4 scale for upper parts of plants, and 0-10 Zeck’s root-knot scale for the roots. When seed bacterization and seed bacterization plus seedling drenching applications compared with positive control; plants infected with root-knot nematodes alone, it was determined that disease symptoms on upper parts of the plants were reduced 35 % and 21 %; gal formation on the roots were reduced 44 % and 39 %,respectively. The experiment has been repeated twice to observe the colonization and population dynamic of antagonistic bacteria on the roots.
Açıklama
Anahtar Kelimeler
Biyoloji
Kaynak
Türkiye Entomoloji Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
30
Sayı
3
