Site-Directed Mutagenesis
Küçük Resim Yok
Tarih
2011
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Humana Press Inc
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The technique of site-directed mutagenesis has been used to characterize gene and protein structure function relationships, protein protein interactions, binding domains of proteins, or active sites of enzymes for the last three decades. In this technique, a nucleotide sequence of interest is experimentally altered using synthetic oligonucleotides. The most commonly used approach is to use an oligonucleotide that is complementary to part of a single-stranded DNA template, but containing an internal mismatch to direct the mutation. In addition to single point mutations, this approach may also be used to construct multiple mutations, insertions, or deletions. As a result of its broad applicability in disease gene characterization studies, numerous commercial kits are now available, making this technique quick, straightforward, and reliable. In this chapter, we detail the steps involved in site-directed mutagenesis and highlight the essentials of this versatile technique based upon our experience.
Açıklama
Anahtar Kelimeler
DNA, Polymerase chain reaction, Site-specific, Mutagenesis, Template, Point mutation, Insertion, Deletion, Amino acid, Oligonucleotide, Restriction site, Efficiency, ssDNA, Transformation
Kaynak
Disease Gene Identification: Methods and Protocols
WoS Q Değeri
N/A
Scopus Q Değeri
N/A
Cilt
700
