Evaluation of a new biosensor-based mushroom (Agaricus bisporus) tissue homogenate: Investigation of certain phenolic compounds and some inhibitor effects
Küçük Resim Yok
Tarih
2004
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
A biosensor based on mushroom tissue homogenate for detecting some phenolic compounds (PCs) and usage of the biosensor for quantifying certain substances that inhibit the polyphenol oxidase activity in mushroom (Agaricus bisporus) tissue homogenate is described. The mushroom tissue homogenate was immobilized to the top of a Clark-type oxygen electrode with gelatin and glutaraldehyde. Optimization of the experimental parameters was done by buffer system, pH, buffer concentration, and temperature. Besides, the detection range of eight phenolic compounds were obtained with the help of the calibration graphs. Thermal stability, storage stability, and repeatability of the biosensor were also investigated. A linear response was observed from 20×10-3 to 200×10-3 mM phenol. The biosensor retained approximately 74% of its original activity after 25 days of storage at 4 °C. In repeatability studies, variation coefficient (C.V.) and standard deviation (S.D.) were calculated as 2.44% and ±0.002, respectively. Inhibition studies revealed that the proposed biosensor was applicable for monitoring benzoic acid and thiourea in soft drinks and fruit juices. © 2004 Elsevier B.V. All rights reserved.
Açıklama
Anahtar Kelimeler
Agaricus bisporus, Biosensor, Mushroom, Phenolic compounds, Polyphenol oxidase
Kaynak
Biosensors and Bioelectronics
WoS Q Değeri
Scopus Q Değeri
Q1
Cilt
20
Sayı
3
