Streptavidin Modified Carbon Nanotube Based Graphite Electrode for Label-Free Sequence Specific DNA Detection

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dc.contributor.authorErdem, Arzum
dc.contributor.authorPapakonstantinou, Pagona
dc.contributor.authorMurphy, Hayley
dc.contributor.authorMcMullan, Martin
dc.contributor.authorKaradeniz, Hakan
dc.contributor.authorSharma, Surbhi
dc.date.accessioned2019-10-27T21:18:43Z
dc.date.available2019-10-27T21:18:43Z
dc.date.issued2010
dc.departmentEge Üniversitesien_US
dc.description.abstractDisposable graphite pencil electrodes (PGE) modified with multiwalled carbon nanotubes (MWCNTs)-streptavidin (STR) conjugates were used for electrochemical monitoring of label-free DNA hybridization. The surface morphology of PGE electrode before and after hybridization was characterized by scanning electron microscopy. Electrochemical impedance spectroscopy was used to monitor each step of the construction of the DNA biosensor. The biosensor was demonstrated to have excellent selectivity, being able to differentiate complementary sequences from a noncomplementary ones and in addition select the target sequence of DNA from a mixture of other DNA without loss in current sensitivity.en_US
dc.description.sponsorshipRoyal Society Joint Project ProgramRoyal Society of London [1212R0168]; Turkish Scientific and Technological Council (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [106S181]; Turkish Academy of Sciences (TUBA)Turkish Academy of Sciencesen_US
dc.description.sponsorshipThis work was supported by the Royal Society Joint Project Program (Project No. 1212R0168). A. E. acknowledges the financial support from the Turkish Scientific and Technological Council (TUBITAK) (Project No. 106S181), and she also would like to express her gratitude to the Turkish Academy of Sciences (TUBA) as an Associate member for their support.en_US
dc.identifier.doi10.1002/elan.200900436
dc.identifier.endpage617en_US
dc.identifier.issn1040-0397
dc.identifier.issn1040-0397en_US
dc.identifier.issue6en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage611en_US
dc.identifier.urihttps://doi.org/10.1002/elan.200900436
dc.identifier.urihttps://hdl.handle.net/11454/44031
dc.identifier.volume22en_US
dc.identifier.wosWOS:000275803200002en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherWiley-V C H Verlag Gmbhen_US
dc.relation.ispartofElectroanalysisen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectMultiwalled carbon nanotubeen_US
dc.subjectBiotin-streptavidinen_US
dc.subjectDNA hybridizationen_US
dc.subjectguanine signalen_US
dc.subjectElectrochemical impedance spectroscopyen_US
dc.subjectNanotubesen_US
dc.titleStreptavidin Modified Carbon Nanotube Based Graphite Electrode for Label-Free Sequence Specific DNA Detectionen_US
dc.typeArticleen_US

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