Streptavidin Modified Carbon Nanotube Based Graphite Electrode for Label-Free Sequence Specific DNA Detection
Küçük Resim Yok
Tarih
2010
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wiley-V C H Verlag Gmbh
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Disposable graphite pencil electrodes (PGE) modified with multiwalled carbon nanotubes (MWCNTs)-streptavidin (STR) conjugates were used for electrochemical monitoring of label-free DNA hybridization. The surface morphology of PGE electrode before and after hybridization was characterized by scanning electron microscopy. Electrochemical impedance spectroscopy was used to monitor each step of the construction of the DNA biosensor. The biosensor was demonstrated to have excellent selectivity, being able to differentiate complementary sequences from a noncomplementary ones and in addition select the target sequence of DNA from a mixture of other DNA without loss in current sensitivity.
Açıklama
Anahtar Kelimeler
Multiwalled carbon nanotube, Biotin-streptavidin, DNA hybridization, guanine signal, Electrochemical impedance spectroscopy, Nanotubes
Kaynak
Electroanalysis
WoS Q Değeri
Q2
Scopus Q Değeri
Q2
Cilt
22
Sayı
6
