An inhibition type alkaline phosphatase biosensor for amperometric determination of caffeine

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dc.contributor.authorAkyilmaz, Erol
dc.contributor.authorTuremis, Mehmet
dc.date.accessioned2019-10-27T21:16:20Z
dc.date.available2019-10-27T21:16:20Z
dc.date.issued2010
dc.departmentEge Üniversitesien_US
dc.description.abstractA biosensor based on the inhibition of alkaline phosphatase (ALP) enzyme was developed for caffeine determination. The biosensor was prepared by a chemical covalent immobilization of alkaline phosphatase with a cross-linking agent, glutaraldehyde on a ceramic based gold screen printed electrode that was modified with cysteamine by forming a self-assembled monolayer. Caffeine competitively inhibits ALP enzyme and the determination method of caffeine by the biosensor was based on this inhibition effect of caffeine. The principle of the measurement was based on the determination of the differentiation of biosensor responses in the enzymatic reaction catalyzed by ALP in the absence and the presence of caffeine. Differences between the biosensor responses were related to caffeine concentration which was added in to the reaction medium. Caffeine concentration can be determined accurately between 0.1 and 10 mu M using the biosensor. Detection limit of the biosensor is 0.08 mu M. In the optimization studies of the biosensor, glycine buffer (pH 10.5; 50 mM) and 30 degrees C were obtained as the optimum working conditions. (C) 2010 Elsevier Ltd. All rights reserved.en_US
dc.identifier.doi10.1016/j.electacta.2010.04.038
dc.identifier.endpage5199en_US
dc.identifier.issn0013-4686
dc.identifier.issn0013-4686en_US
dc.identifier.issue18en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage5195en_US
dc.identifier.urihttps://doi.org/10.1016/j.electacta.2010.04.038
dc.identifier.urihttps://hdl.handle.net/11454/43656
dc.identifier.volume55en_US
dc.identifier.wosWOS:000279215500021en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherPergamon-Elsevier Science Ltden_US
dc.relation.ispartofElectrochimica Actaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBiosensoren_US
dc.subjectCaffeineen_US
dc.subjectSelf-assembled monolayeren_US
dc.subjectAlkaline phosphataseen_US
dc.subjectp-Nitrophenyl phosphateen_US
dc.titleAn inhibition type alkaline phosphatase biosensor for amperometric determination of caffeineen_US
dc.typeArticleen_US

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