Significance of microRNA-330-5p/TYMS Expression Axis in the Pathogenesis of Colorectal Tumorigenesis
Küçük Resim Yok
Tarih
2022
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Springer
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Background: Colorectal cancer (CRC) is one of the most common types of cancer worldwide. A number of dysregulated microRNAs (miRNAs) have been linked to CRC progression and treatment response and are thought to be promising prognostic biomarkers for this cancer. microRNA-330 (miR-330-5p) has been reported to inhibit cell proliferation through suppressing thymidylate synthase (TYMS). In the current study, miR-330-5p, TYMS, and their interactions were investigated to evaluate their therapeutic and diagnostic value for CRC treatment. Methods: The expression levels of miR-330-5p and TYMS were evaluated in silico using TCGA datasets for CRC. Data validation was performed on a set of internal samples (100 pairs of CRC tumor specimens and adjacent non-cancerous samples) utilizing real-time PCR assay. The linkage between clinicopathological parameters and expression levels was also investigated. Results: TCGA results indicated that miR-330-5p and TYMS were significantly upregulated and downregulated in the CRC, respectively. Real-time PCR results confirmed that the expression of miR-330-5p was significantly upregulated in tumor tissues relative to marginal tissues (P = 0.0005), whereas TYMS expression was significantly downregulated (P = 0.0001). The transcript level of miR-330-5p was associated with tumor stage and lymph node metastases. Conclusion: The microRNA-330 inhibited cell proliferation by suppressing thymidylate synthase (TYMS) in colorectal cancer. Therefore, suggesting that they are valuable factors for further studies of alternative treatment and diagnostic methods. © 2021, Springer Science+Business Media, LLC, part of Springer Nature.
Açıklama
Anahtar Kelimeler
Colorectal cancer, MicroRNA, miR-330-5p, Prognosis, TYMS, microRNA, microRNA 330 5p, thymidylate synthase, unclassified drug, microRNA, MIRN330 microRNA, human, thymidylate synthase, TYMS protein, human, adult, alternative medicine, Article, cancer staging, cancer therapy, cancer tissue, carcinogenesis, cell proliferation, cohort analysis, colorectal cancer, computer model, controlled study, diagnostic test accuracy study, diagnostic value, down regulation, female, gene expression, human, human tissue, lymph node metastasis, major clinical study, male, middle aged, pathology, protein expression, protein RNA binding, real time polymerase chain reaction, RNA transcription, upregulation, carcinogenesis, cell transformation, colorectal tumor, gene expression regulation, genetics, metabolism, tumor cell line, Carcinogenesis, Cell Line, Tumor, Cell Proliferation, Cell Transformation, Neoplastic, Colorectal Neoplasms, Gene Expression Regulation, Neoplastic, Humans, MicroRNAs, Thymidylate Synthase
Kaynak
Journal of Gastrointestinal Cancer
WoS Q Değeri
Scopus Q Değeri
N/A
Cilt
53
Sayı
4
