Gingival crevicular fluid and salivary HIF-1?, VEGF, and TNF-? levels in periodontal health and disease
Küçük Resim Yok
Tarih
2019
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wiley-Blackwell
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Background: Hypoxia-inducible factor-1 alpha (HIF-1?) is expressed as an adaptive response to hypoxia, mediates angiogenesis through the expression of vascular endothelial growth factor (VEGF) and can be induced by tumor necrosis factor-alpha (TNF-?). This study aimed to investigate the gingival crevicular fluid (GCF) and salivary HIF-1?, VEGF, and TNF-? levels in periodontal health and disease. Methods: A total of 87 individuals, 20 generalized aggressive periodontitis (G-AgP), 20 chronic periodontitis (CP), 26 gingivitis patients, and 21 periodontally healthy individuals, were included. Clinical periodontal parameters were recorded; GCF and salivary samples were collected; and HIF-1?, VEGF, and TNF-? levels were measured by enzyme-linked immunosorbent assay. Nonparametric tests were used for the statistical analyses. Results: G-AgP and CP groups had significantly higher GCF HIF-1?, VEGF, and TNF-? total amounts than gingivitis and healthy groups (P < 0.05). GCF HIF-1? and TNF-? total amounts in gingivitis group were significantly higher than the healthy group (P < 0.05). GCF and salivary concentrations of biomarkers were similar in both periodontitis groups (P > 0.05). Salivary HIF-1? concentrations in gingivitis group were significantly higher than G-AgP and healthy groups (P < 0.05). GCF HIF-1?, VEGF, and TNF-? total amounts were positively correlated with the site-specific clinical periodontal parameters and with each other (P < 0.05). Conclusions: HIF-1? is detectable in GCF and saliva of periodontally diseased and healthy individuals, and the GCF levels of the biomarker can be affected by disease status. Increased GCF HIF-1?, VEGF, and TNF-? levels in both chronic and aggressive form of periodontitis might suggest the role of TNF-?/HIF-1?/VEGF pathway in the pathogenesis of periodontal diseases. © 2018 American Academy of Periodontology
Açıklama
Anahtar Kelimeler
cytokines, gingival crevicular fluid, periodontitis, saliva
Kaynak
Journal of Periodontology
WoS Q Değeri
Scopus Q Değeri
Q1
Cilt
90
Sayı
7
