Sıçanlarda akut asetaminofen nefrotoksisitesi ve üriner gamma-glutamil transferaz aktivitesi
Küçük Resim Yok
Tarih
1997
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Asetaminofen nefrotoksisitesi ve üriner gamma-glutamil transferaz (GGT) atılımı arasındaki ilişkiyi araştırmak için sıçanlara 900 mg/kg asetaminofen (APAP) tek doz olarak i.p. yoldan uygulanmıştır. İlaç uygulamasını takiben sıçanların 24 saatlik idrarları toplanmış ve böbrekler eter anestezisi altında çıkartılarak histolojik olarak incelemeye alınmıştır. 24 saatlik idrar örneklerinde GGT aktivite ölçümleri ve kreatinin miktar tayinleri yapılmıştır. APAP uygulanan grubun (n= 12) üriner GGT aktivitesi (1.88 ± 0.21 U/mg kreatinin), kontrol grubundan fn= 16) (0.77 ± 0.05 U/mg kreatinin) anlamlı olarak yüksek bulunmuştur (p< 0.0002). Işık mikroskobu kullanılarak yapılan histolojik incelemelerde, böbrek dokusundaki değişikliklerin minimal düzeyde olduğu gözlenmiş ve deneysel nefrotoksisite çalışmalarında üriner GGT aktivite ölçümlerinin yararı tartışılmıştır.
In order to determine the relation between the nephrotoxicity of acetaminophen and urinary gamma-glutamyl transferase (GGT) excretion, single dose of 900 mg/kg acetaminophen (APAP) was administered to the rats intrapehtoneally. Following drug administration, 24 hour urine of the rats was collected and their kidneys were removed under aether anesthesia for histological examinations. GGT activity measurements and quantitative analysis for creatinine has been carried out on urine samples. Urinary GGT activity in APAP administered group (n- 12) (1.88 ± 0.21 U/mg creatinine) was found to-be significantly higher than the control group (n= 16) (0.77 ± 0.05 U/mg creatinine) (p< 0,0002). His¬tological examination of the kidneys under light microscopy exhibited that the degree of tissue differentiation was at minimal level and the use of urinary GGT activity measurements in experimental nephrotoxicity studies has been dis¬cussed.
In order to determine the relation between the nephrotoxicity of acetaminophen and urinary gamma-glutamyl transferase (GGT) excretion, single dose of 900 mg/kg acetaminophen (APAP) was administered to the rats intrapehtoneally. Following drug administration, 24 hour urine of the rats was collected and their kidneys were removed under aether anesthesia for histological examinations. GGT activity measurements and quantitative analysis for creatinine has been carried out on urine samples. Urinary GGT activity in APAP administered group (n- 12) (1.88 ± 0.21 U/mg creatinine) was found to-be significantly higher than the control group (n= 16) (0.77 ± 0.05 U/mg creatinine) (p< 0,0002). His¬tological examination of the kidneys under light microscopy exhibited that the degree of tissue differentiation was at minimal level and the use of urinary GGT activity measurements in experimental nephrotoxicity studies has been dis¬cussed.
Açıklama
Anahtar Kelimeler
Cerrahi
Kaynak
Ege Tıp Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
36
Sayı
1-2
