Cytokine (interleukin-1beta) and MMP levels in gingival crevicular fluid after use of platelet-rich fibrin or connective tissue graft in the treatment of localized gingival recessions
Küçük Resim Yok
Tarih
2016
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wiley
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Background and ObjectiveThe objective of this study was to evaluate the levels of MMP-8, MMP-9, TIMP-1 and interleukin-1beta (IL-1) in gingival crevicular fluid during the early and late stages of healing in gingival recession sites treated with coronally advanced flap plus platelet-rich fibrin (CAF+PRF) compared with CAF plus connective tissue graft (CAF+CTG). Material and MethodsTwenty-four nonsmoking patients with Miller Class I or Class II localized gingival recession defects in bilateral sites received treatment with either CAF+PRF (PRF group) or CAF+CTG (CTG group). Gingival crevicular fluid samples were collected at baseline and at 10d and 1mo, 3mo and 6mo after surgery. The levels of MMP-8, MMP-9, TIMP-1 and IL-1 in gingival crevicular fluid were measured using a time-resolved immunofluorometric assay and ELISAs. ResultsGingival crevicular fluid levels of IL-1 were significantly elevated in the CTG group at 10d compared with baseline (p<0.05). At 10d after surgery, the levels of TIMP-1 in gingival crevicular fluid showed a significant decrease in the CTG group compared with the PRF group (p<0.05). The levels of IL-1 and MMP-8 in gingival crevicular fluid were significantly lower in the PRF group than in the CTG group at 10d (p<0.05). No significant differences were found in all clinical and biochemical parameters at 1, 3, and 6mo between study groups (p>0.05). ConclusionRoot coverage with CAF+PRF has a significant effect on increasing gingival crevicular fluid TIMP-1 levels and suppressing gingival crevicular fluid MMP-8 and IL- levels at 10d. Gingival crevicular fluid levels of MMP-8, MMP-9, TIMP-1 and IL-1 did not seem to be affected by the technique at later phases of wound healing.
Açıklama
Anahtar Kelimeler
connective tissue, cytokine, fibrin, gingival crevicular fluid, gingival recession, matrix metalloproteinases
Kaynak
Journal of Periodontal Research
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
51
Sayı
4
