Comparison of chitin and amberlite IRA-938 for ?-galactosidase immobilization
Küçük Resim Yok
Tarih
2003
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Watermelon ?-galactosidase (EC 3.2.1.22) was immobilized on a natural (chitin) and a synthetic anion-exchange (Amberlite IRA-938) support by covalent coupling methods. The procedure entails the activation of supports with 1,1'-carbonyldiimidazole (CDI), followed by immobilization of the enzyme on to these supports without and with a spacer arm; ?-aminobutyric acid (GABA). Optimization of activation was performed by changing the CDI concentrations and coupling efficiencies. The comparison of two immobilization techniques for both chitin and Amberlite IRA-938 was made by comparing different enzyme concentrations against enzyme activity yield. Furthermore, the storage stability of the immobilized enzymes was also investigated and chitin immobilized ?-galactosidase was found to be better. Although the activity yield of immobilized enzymes were the same for both supports, the short storage stability of immobilized enzyme on Amberlite IRA-938 is currently a drawback to its applications.
Açıklama
Anahtar Kelimeler
?-Galactosidase, Amberlite IRA-938, Chitin, Enzyme immobilization, Watermelon (Citrullus vulgaris)
Kaynak
Artificial Cells, Blood Substitutes, and Immobilization Biotechnology
WoS Q Değeri
Scopus Q Değeri
N/A
Cilt
31
Sayı
1
