Sulfite Determination by a Biosensor Based on Bay Leaf Tissue Homogenate: Very Simple and Economical Method
Küçük Resim Yok
Tarih
2009
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Taylor & Francis Inc
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Of all the food additives for which the FDA has received adverse reaction reports, the ones that most closely resemble true allergens are sulfur-based preservatives. Sulfites are used primarily as antioxidants to prevent or reduce discoloration of light-colored fruits and vegetables, such as dried apples and potatoes, and to inhibit the growth of microorganisms in fermented foods such as wine. This work aims to prepare an electrochemical biosensor based on bay leaf tissue homogenate that contains polyphenol oxidase enzyme abundantly for sulfite detection in foods. The principle of the biosensor is based on the inhibition effect of sulfites on polyphenol oxidase in the bioactive layer. Optimum conditions for the biosensor, such as temperature and pH, were investigated. Some stability parameters of the biosensor were also identified. The biosensor showed a linear calibration graph in the range of 25-100 M sulfite. The biosensor presents a very simple, economical, reliable, and feasible method for sulfite detection in foods.
Açıklama
Anahtar Kelimeler
Sulfite, food additives, biosensor, allergic additives
Kaynak
Artificial Cells Blood Substitutes and Biotechnology
WoS Q Değeri
Q4
Scopus Q Değeri
N/A
Cilt
37
Sayı
3
