Granulocyte-colony stimulating factor decreases the extent of ovarian damage caused by cisplatin in an experimental rat model
Küçük Resim Yok
Tarih
2014
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Korean Soc Gynecology Oncology & Colposcopy
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Objective: To investigate whether granulocyte-colony stimulating factor (G-CSF) can decrease the extent of ovarian follicle loss caused by cisplatin treatment. Methods: Twenty-one adult female Sprague-Dawley rats were used. Fourteen rats were administered 2 mg/kg/day cisplatin by intraperitoneal injection twice per week for five weeks (total of 20 mg/kg). Half of the rats (n=7) were treated with 1 mL/kg/day physiological saline, and the other half (n=7) were treated with 100 mu g/kg/day G-CSF. The remaining rats (n=7, control group) received no therapy. The animals were then euthanized, and both ovaries were obtained from all animals, fixed in 10% formalin, and stored at 4 degrees C for paraffin sectioning. Blood samples were collected by cardiac puncture and stored at 30 degrees C for hormone assays. Results: All follicle counts (primordial, primary, secondary, and tertiary) and serum anti-Mullerian hormone levels were significantly increased in the cisplatin+G-CSF group compared to the cisplatin+physiological saline group. Conclusion: G-CSF was beneficial in decreasing the severity of follicle loss in an experimental rat model of cisplatin chemotherapy.
Açıklama
Anahtar Kelimeler
Anti-Mullerian hormone, Cisplatin, Granulocyte-colony stimulating factor, Ovarian follicle, Ovary
Kaynak
Journal of Gynecologic Oncology
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
25
Sayı
4
