Adjuvant effects of novel water/oil emulsion formulations on immune responses against infectious bronchitis (IB) vaccine in mice
Küçük Resim Yok
Tarih
2024
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Academic Press
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Vaccines have long made use of adjuvants to boost the immune response of the body and reduce the amount of vaccine needed as well as the expense of producing the vaccine. Many vaccine adjuvants are in development, but their application in veterinary vaccinations is restricted due to their lack of efficacy or undesirable side effects. For this reason, it is essential to develop novel adjuvants. To address the issue that the currently available infectious bronchitis (IB) vaccine often fails to produce sufficient immune responses, Coral Biotechnology tested two of their newly developed water-in-oil (W/O) type emulsion adjuvants (Coralvac RZ 528 and Coralvac RZ 506) in the IB vaccine. These adjuvants were tested in a mouse model to determine whether it worked with an inactive IBV H120 vaccine. Vaccine formulations were prepared by combining a virus concentration of 1 × 106 EID50/0.1 ml with an emulsion of the W/O type in a specific ratio. Once the formulations were ready, it was injected intramuscularly as a single dosage, and the mice were monitored for 21 days afterwards. The results showed that anti-IB antibody titer (IgG and IgG1), CD3+ CD8+ T cell responses as well as IFN- ? cytokine production, and splenocyte proliferation were all considerably higher in the IBV H120 with Coralvac RZ 528 and IBV H120 with Coralvac RZ 506 formulation groups than in the viral control group. According to our findings, the humoral and cellular immune responses of mice were significantly enhanced by these novel vaccine adjuvants. Thus, our results provide evidence that the W/O type emulsion adjuvants developed by Coral Biotechnology may be a useful adjuvant in IBV vaccines. © 2023 International Alliance for Biological Standardization
Açıklama
Anahtar Kelimeler
Adjuvant, Immune response, Infectious bronchitis, Vaccine, Water-in-oil (W/O), avian infectious bronchitis virus vaccine, gamma interferon, immunoglobulin G antibody, immunoglobulin G1 antibody, interleukin 1beta, interleukin 4, vaccine adjuvant, water oil cream, animal experiment, animal model, antibody titer, Article, avian infectious bronchitis, Avian infectious bronchitis virus, CD3+ T lymphocyte, CD8+ T lymphocyte, cell proliferation, cellular immunity, controlled study, cytokine production, drug efficacy, drug formulation, embryo, emulsion, female, humoral immunity, immune response, LD50, male, mouse, mouse model, nonhuman, single drug dose, spleen cell, Turkey (republic), vaccination, virus inactivation, virus strain
Kaynak
Biologicals
WoS Q Değeri
Scopus Q Değeri
Q2
Cilt
85
