Electrochemical DNA biosensor for the detection of specific gene related to Microcystis species
Küçük Resim Yok
Tarih
2001
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier Science Inc
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
A new electrochemical biosensor is described for voltammetric detection of gene sequence related to bloom-forming genera of cyanobacteria, Microcystis spp. The sensor involves the immobilization of a 17-mer DNA probe, which is complementary to a specific gene sequence related to Microcystis spp. on a gold electrode through specific adsorption. The DNA probe was used to determine the amount of target gene in solution using methylene blue (MB) and ruthenium bipyridine as the electrochemical indicators. The anodic peak currents (i(pa)) of Ru(bpy)(3)(2+) were linearly related to the concentration of the target oligonucleotide sequence in the range 1.8 x 10(-10) -9.0 x 10(-8) M. The detection limit of this approach was 9.0 x 10(-11) M. In addition, these indicators were capable of selectively discriminating against mismatches: a very desirable condition for the detection of disease-related point-mutation in guanine bases of the cyanobacteria. (C) 2001 Published by Elsevier Science B.V.
Açıklama
Anahtar Kelimeler
Microcystis spp., DNA hybridization, methylene blue, ruthenium bipyridine
Kaynak
Electrochemistry Communications
WoS Q Değeri
Q2
Scopus Q Değeri
Q1
Cilt
3
Sayı
5
