p(AAm/TA)-based IPN hydrogel films with antimicrobial and antioxidant properties for biomedical applications

Interpenetrating polymer networks (IPN), either semi-IPN (s-IPN) or full IPN, based on a natural polymer tannic acid (TA) and synthetic poly(acrylamide) (p(AAm)) were prepared by incorporation of TA during p(AAm) hydrogel film preparation with and without crosslinking of TA simultaneously. The synthesis of p(AAm/TA) s-IPN and IPN hydrogels with different amounts of TA were prepared by concurrent use of redox polymerization and epoxy crosslinking. The p(AAm)-based hydrogels were completely degraded at 37.5 degrees C within 9 and 2 days at pHs 7.4 and 9, respectively. Biocompatibility of p(AAm), s-IPN, and IPN were tested with WST assay and double staining, they had 75% cell viability up to almost 20 mu g mL(-1) concentration against L929 fibroblast cell. Antioxidant properties of IPN and s-IPN hydrogels were investigated with a