Synthetic antibodies for methamphetamine analysis: Design of high affinity aptamers and their use in electrochemical biosensors
Küçük Resim Yok
Tarih
2022
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier Science Sa
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
An aptamer-based electrochemical biosensor was designed and fabricated for methamphetamine (METH) anal-ysis. Several aptamer sequences developed by the GO-SELEX method were immobilized onto gold electrodes (GE) via gold-thiol affinity. Surface characterizations were performed by electrochemical methods including cyclic voltammetry (CV), differential pulse voltammetry (DPV), and electrochemical impedance spectrometry (EIS). GE/Apta-4/METH aptasensor was selected for further studies since the best results were obtained with the Apta-4 sequence in terms of analytical parameters. Accordingly, it exhibited good selectivity for METH analysis with a wide linear range of 0.1-50 ng/mL and a low limit of detection (LOD) which was found to be 0.467 ng/mL. The proposed aptasensor platform displayed good selectivity for the detection of METH in synthetic urine samples spiked with METH.
Açıklama
Anahtar Kelimeler
Abused drugs, Methamphetamine, Biosensors, Electrochemical biosensors, Aptasensors, In-Vitro Selection, Exponential Enrichment, Systematic Evolution, Drug, Microextraction, Strategies, Molecules, Ligands, Binding, Selex
Kaynak
Journal Of Electroanalytical Chemistry
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
921
