Yazar "Karacali, S" seçeneğine göre listele
Listeleniyor 1 - 13 / 13
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe Biochemical and morphological characteristics of selenite-induced apoptosis in human hepatoma Hep G2 cells(Humana Press Inc, 2004) Celik, HA; Aydin, HH; Deveci, R; Terzioglu, E; Karacali, S; Saydam, G.; Akarca, U; Batur, YSelenium is a cellular growth inhibitor in many mammary tumor cells. To comprehend the mechanism for the selenium-induced cell death, we examined the effects of sodium selenite, which has been one of the most extensively investigated selenium compounds, in human hepatoma Hep G2 cells. Cell viability gradually decreased after treatment with sodium selenite within the concentration range of 10-50 muM. Low (10 muM) selenite has shown a high-percentage laddering pattern compared to the high (25 muM) cytotoxic selenium concentration in agarose gel electrophoresis. G(2)/M-phase enrichment was also concentration dependent. The most consistent transmission electron microscopic finding was the existence of large lysosomes. Based on these data, we hypothesize that sodium selenite predominantly shows its apoptotic effect over hydrogen selenite accumulation.Öğe Characterization of the cellular response during apoptosis induction in cadmium-treated Hep G2 human hepatoma cells(Humana Press Inc, 2003) Aydin, HH; Celik, HA; Deveci, R; Terzioglu, E; Karacali, S; Mete, N; Akarca, U; Batur, YCadmium is a toxic transition heavy metal of continuing occupational and environmental concern, with a wide variety of adverse effects on regulation of gene expression and cellular signal transduction pathways. Injury to cells by cadmium leads to a complex series of events that can culminate in the death of the cell. It has been reported that cadmium induces apoptosis in many cell lines. However, the morphological characteristics leading to apoptosis or subsequent regeneration in cells exposed to cadmium have not been clarified. We evaluated whether human hepatoma cells maintained in culture undergo apoptosis when exposed to cadmium. Cytotoxic activity of cadmium on Hep G2 cells determined using 3-[4,5-dimethylthiazol-2-yl]-2,5diphenyltetrazolium bromide assay. A DNA ladder assay was performed by electrophoresis. Cell cycle analysis was quantified by flow cytometry. Nuclear morphology was studied by fluorescence microscopy after staining with propidium iodide and Hoechst 33342. Morphologic alterations in culture hepatocytes treated with CdCl2 were observed by transmission electron microscopy. We have demonstrated that apoptosis is a major mode of elimination of damaged HepG2 cells in cadmium toxicity and it precedes necrosis.Öğe Characterization of the cellular response during apoptosis induction in cadmium-treated Hep G2 human hepatoma cells(Humana Press Inc, 2003) Aydin, HH; Celik, HA; Deveci, R; Terzioglu, E; Karacali, S; Mete, N; Akarca, U; Batur, YCadmium is a toxic transition heavy metal of continuing occupational and environmental concern, with a wide variety of adverse effects on regulation of gene expression and cellular signal transduction pathways. Injury to cells by cadmium leads to a complex series of events that can culminate in the death of the cell. It has been reported that cadmium induces apoptosis in many cell lines. However, the morphological characteristics leading to apoptosis or subsequent regeneration in cells exposed to cadmium have not been clarified. We evaluated whether human hepatoma cells maintained in culture undergo apoptosis when exposed to cadmium. Cytotoxic activity of cadmium on Hep G2 cells determined using 3-[4,5-dimethylthiazol-2-yl]-2,5diphenyltetrazolium bromide assay. A DNA ladder assay was performed by electrophoresis. Cell cycle analysis was quantified by flow cytometry. Nuclear morphology was studied by fluorescence microscopy after staining with propidium iodide and Hoechst 33342. Morphologic alterations in culture hepatocytes treated with CdCl2 were observed by transmission electron microscopy. We have demonstrated that apoptosis is a major mode of elimination of damaged HepG2 cells in cadmium toxicity and it precedes necrosis.Öğe Fine-Structure of the Prothoracic Glands During the Last Larval Instar In Spodoptera-Littoralis (Bois) (Lepidoptera)(Academic Press Inc Jnl-Comp Subscriptions, 1980) Karacali, SÖğe Glycobiology - Current molecular biology(Scientific Technical Research Council Turkey, 2003) Karacali, SCarbohydrate chemistry evolved into carbohydrate biochemistry and gradually into the biology of carbohydrates, or glycobiology, at the end of the last century. Glycobiology is the new research area of modern molecular biology, and it investigates the structure, biosynthesis and biological functions of glycans. The numbers, linkage types (alpha or beta), positions, binding points and functional group differences of monosaccharides create microheterogeneity. Thus, numerous glycoforms with precise structural differences occur. Each glycoform as a distinct biological ligand has a different function. Studies in glycobiology have concentrated on determining molecular structures and glycosylation mechanisms, investigating biological control mechanisms and the phenotypic characteristics of different cell types and on the cloning of related enzymes. Glycobiology is of increasing importance in fundamental research, medical science and modern biotechnology.Öğe Griscelli syndrome: Report of a case and review of the literature(Blackwell Publishing Asia, 2001) Kurugol, Z; Özkınay, F; Vardar, F; Karacali, S; Kutukculer, N; Deveci, RÖğe Neurosecretory-System of Adult Melanogryllus-Desertus (Orthoptera, Gryllidae) - Intracellular Microorganisms In the Median Neurosecretory and Glial-Cells(Academic Press Inc Jnl-Comp Subscriptions, 1982) Karacali, SÖğe The Neurosecretory-System of the Adult Melanogryllus-Desertus Pall (Orthoptera, Gryllidae) .1. Ultrastructural-Study of the Median Neurosecretory-Cells In the Brain(Springer, 1980) Karacali, S; Geldiay, SÖğe The Neurosecretory-System of the Adult Melanogryllus-Desertus Pall (Orthoptera, Gryllidae) .2. Cerebral Neurohemal Area(Springer Verlag, 1980) Geldiay, S; Karacali, SÖğe The Neurosecretory-System of the Adult Melanogryllus-Desertus Pall (Orthoptera, Gryllidae) .3. Crystalline Pattern of Neurosecretory Material(Academic Press Inc Jnl-Comp Subscriptions, 1982) Geldiay, S; Karacali, SÖğe Rhabdome-Bearing Cells Within the Corpus Cardiacum of Melanogryllus-Desertus (Orthoptera, Gryllidae)(Academic Press Inc Elsevier Science, 1983) Geldiay, S; Karacali, SÖğe Rhabdoviruslike Particles In Corpus-Cardiacum and Corpus-Allatum of Melanogryllus-Desertus (Orthoptera, Gryllidae)(Academic Press Inc Jnl-Comp Subscriptions, 1994) Karacali, SÖğe Storage of Rat-Liver For Plasma-Membrane Isolation(Academic Press Aust, 1995) Tanyalcin, T; Deveci, R; Kutay, F; Karacali, S; Sozmen, EyWe described a procedure for the preservation of rat liver which makes possible the isolation of plasma membranes after 10 days storage at -70 degrees C. The yield of plasma membranes obtained from the liver tissue kept at -70 degrees C for 10 days (3.43 +/- 0.08 mg protein/10 g wet liver) was not different statistically (P > 0.05) from the yield of freshly obtained plasma membranes (3.32 +/- 0.05 mg protein/10 g wet liver). However, a significantly low yield (2.65 +/- 0.08; P < 0.01) was obtained from 90 days stored rat liver when compared with the immediate isolation. Plasma membrane Na+, K+ ATPase and 5'nucleotidase activities of the stored liver for 10 days were not different statistically (P > 0.05) from the enzyme activities of the freshly isolated membrane fractions. In contrast there was a significant decrease (p < 0.0001) in the activities of both plasma membrane Na+, K+ ATPase and 5'nucleotidase activities of 90 days stored rat liver at -70 degrees C when compared with immediate isolation. Considering the electron microscopic findings; we observed that the preservation of the integrity of the plasma membrane fractions obtained from fresh and frozen livers for 10 and 90 days seemed to be parallel to the biochemical results. Therefore we suggest that, storage of rat liver tissue for 10 days make feasible to maintain the experimental design and give convenience for obtaining intact plasma membrane fractions.