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    Effects of broccoli extract on biodistribution and labeling blood components with Tc-99m-GH
    (Acta Cirurgica Brasileira, 2011) Cekic, Betul; Muftuler, Fazilet Zumrut Biber; Kilcar, Ayfer Yurt; Ichedef, Cigdem; Unak, Perihan
    Purpose: People consume vegetables without the knowledge of the side effects of the biological and chemical contents and interactions between radiopharmaceuticals and herbal extract. To this end, current study is focused on the effects of broccoli extract on biodistribution of radiolabeled glucoheptonate (Tc-99m-GH) and radiolabeling of blood components. Methods: GH was labeled with Tc-99m. Quality control studies were done utilizing TLC method. Biodistribution studies were performed on male rats which were treated via gavage with either broccoli extract or SF as control group for 15 days. Blood samples were withdrawn from rats' heart. Radiolabeling of blood constituents performed incubating with GH, SnCl2 and Tc-99m. Results: Radiochemical yield of Tc-99m-GH is 98.46 +/- 1.48 % (n=8). Biodistribution studies have shown that according to the control, the treated group with broccoli has approximately 10 times less uptake in kidney. The percentage of the radioactivity ratios of the blood components is found to be same in both groups. Conclusions: Although there is no considerable effect on the radiolabeling of blood components, there is an outstanding change on the biodistribution studies especially on kidneys. The knowledge of this change on kidney uptake may contribute to reduce the risk of misdiagnosis and/or repetition of the examinations in Nuclear Medicine.
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    In vitro evaluation of radiolabeled (I-125) methanol extracts of yarrow in cell lines of MCF-7, PC-3, A-549 and Caco-2
    (Springer, 2013) Kilcar, Ayfer Yurt; Cekic, Betul; Muftuler, Fazilet Zumrut Biber; Unak, Perihan; Medine, Emin Ilker
    Nowadays, cancer is still the second leading cause of death all over the world. Therefore, natural products which have anticancer and antitumor properties are come into prominence. Achillea family is known with anticancer and antitumor activity. Yarrow which has over a hundred bioactive compounds is a member of Achillea family. In current study; components of yarrow which was obtained after methanol extraction and purification were radiolabeled with I-125 and effects of these radiolabeled components on the cells were examined with using Caco-2, MCF-7, A-549, PC-3 cell lines. As a result of these studies, seven peaks were obtained and the highest radiolabeling yield was calculated for I-125 radiolabeled Peak 7 (95.00 +/- A 7.07, n = 4). To screen the biological properties of these radiolabeled peaks at determined cell lines, our ongoing effort was to evaluate incorporation percentage with time dependent. Furthermore, I-125-Peak 7 had highest incorporation ratio for whole cell lines and its incorporation percentage was increased with time dependent. Results of these in vitro studies were compatible with previous in vivo studies and traditional use of yarrow plants.
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    Interaction between green tea extract and Tc-99m-pertechnetate on in vivo distribution
    (Springer, 2014) Sabuncu, Burak; Muftuler, Fazilet Zumrut Biber; Kilcar, Ayfer Yurt; Cekic, Betul; Ucar, Eser; Unak, Perihan
    People drink various types of tea without knowing the side effects of biological and chemical contents and radiopharmaceutical interactions. In current study, it is aimed to evaluate the effects of green tea extract in different extraction solvents on the radiolabeling of the blood constituents with Tc-99m and on the biodistribution of radiopharmaceutical sodium pertechnetate ((NaTcO4)-Tc-99m) in male Wistar Albino rats. The extraction of green tea was performed in different solvents. Biodistribution studies were performed on male rats which were treated via gavage with green tea extract in different extraction solvents or saline (0.9 % NaCl) as a control group for 7 days. The radiolabeling of blood constituents performed incubating with SnCl2 and Tc-99m. According to experimental results, radiolabeling blood components with Tc-99m were not modified in the usage of the different extraction solvents for green tea extraction, but a significant alteration (P < 0.05) of biodistribution of (NaTcO4)-Tc-99m was observed after treatment with green tea extract in distilled water. Although there is no considerable effect on radiolabeling of blood components, there is an outstanding change on the biodistribution studies especially with green tea extract in distilled water. The identified change monitored in this study may cause to reduce the risk of misdiagnosis and/or avoid the repetition of the examinations in nuclear medicine.