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Öğe Agresif ve kronik periodontitisli hastalarda cerrahisiz periodontal tedaviye ek olarak kullanılan Azitromisin'in etkisinin klinik, biyokimyasal ve mikrobiyolojik olarak değerlendirilmesi(Ege Üniversitesi, 2008) Baylas, Haluk; Atilla, Gül; Emingil, Gülnur; Sayğan (Han), BuketAggressive periodontitis, chronic periodontitis, MMP-8, TIMP-1, microbiology.;Agresif periodontitis, kronik periodontitis, MMP-8, TIMP-1, azitromisin, mikrobiyoloji.Öğe Angiotensin-converting enzyme (ACE), angiotensinogen (AGT), and angiotensin II type 1 receptor (AT1R) gene polymorphisms in generalized aggressive periodontitis(Pergamon-Elsevier Science Ltd, 2009) Gurkan, Ali; Emingil, Guelnur; Saygan, Buket Han; Atilla, Guel; Kose, Timur; Baylas, Haluk; Berdeli, AfigAim: Host response to periodontopathic microorganisms can be modulated by genetic factors. Accumulated evidence highlighted the role of renin-angiotensin system (RAS) in inflammatory response thus potential implication of this molecular system in the pathogenesis of periodontitis can be suggested. The present study investigated common genetic variants of molecules within the RAS family namely angiotensin-converting enzyme (ACE), angiotensinogen (AGT) and angiotensin 11 type 1 receptor (AT1R) in relation to generalized aggressive periodontitis (G-AgP). Methods: DNA was obtained from peripheral blood of 103 G-AgP patients and 100 periodontally healthy subjects. ACE I/D, AGT M235T and AT1R A1166C polymorphisms were genotyped by polymerase chain reaction and restriction fragment length polymorphism method. Chi-square, ANOVA and logistic regression were used in statistical analyses. Results: Both ACE I/D and ATIR polymorphisms were similar in G-AgP and healthy groups (p>0.05). G-AgP subjects exhibited decreased ACT TT genotype and T allele frequency as compared to healthy subjects (p<0.05). The same trend was also observed in the nonsmoker subgroup regarding investigated RAS polymorphisms. Conclusions: Present findings suggest that AGT M235T TT genotype and T allele might be associated with decreased risk for G-AgP in Turkish population. (C) 2009 Elsevier Ltd. All rights reserved.Öğe Azithromycin as an Adjunctive Treatment of Generalized Severe Chronic Periodontitis: Clinical, Microbiologic, and Biochemical Parameters(Wiley, 2012) Han, Buket; Emingil, Gulnur; Ozdemir, Guven; Tervahartiala, Taina; Vural, Caner; Atilla, Gul; Baylas, Haluk; Sorsa, TimoBackground: This study examines the efficacy of azithromycin in combination with non-surgical periodontal therapy on clinical and microbiologic parameters and gingival crevicular fluid (GCF) matrix metalloproteinases-8 (MMP-8) levels over 6 months in patients with severe generalized chronic periodontitis (CP). Methods: Twenty-eight of 36 patients with severe generalized CP were included in this randomized, double-masked, placebo-controlled, parallel-arm study. They were randomly assigned to azithromycin or placebo groups (500 mg, once daily for 3 days). Probing depth (PD), clinical attachment level, dichotomous presence or absence of supragingival plaque accumulation, and bleeding on probing were recorded. GCF samples were obtained from one single-rooted tooth with PD >= 6 mm, whereas microbiologic samples were collected from two single-rooted teeth with PD >= 6 mm. Microbiologic parameters were analyzed by quantitative real-time polymerase chain reaction for Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia, Fusobacterium nucleatum, Prevotella intermedia, and total bacteria. GCF MMP-8 levels were determined by immunofluorescence assay. Results: Azithromycin and placebo groups demonstrated similar but significant improvements in all clinical parameters (P < 0.05). A. actinomycetemcomitans, P. gingivalis, T. forsythia, P. intermedia, and total bacteria significantly decreased over the 6-month period in both groups, whereas F. nucleatum was significantly reduced in all visits in the azithromycin group, with the levels also being lower compared with those of the placebo group (P < 0.05). The azithromycin and placebo groups exhibited significant reduction in GCF MMP-8 levels at the post-treatment visit and at 2 weeks (P < 0.05). Conclusion: On the basis of the present findings, it can be concluded that adjunctive azithromycin provides no additional benefit over non-surgical periodontal treatment on parameters investigated in patients with severe generalized CP. J Periodontol 2012;83:1480-1491.Öğe Cerrahisiz periodontal tedaviye ek olarak kullanılan meloksikamın dişeti oluğu sıvısı IL-4, IL-6, IFN-gama ve TGF-Beta1 seviyeleri üzerine etkisinin değerlendirilmesi(2001) Buduneli, Nurcan Gülsüm; Vardar, Saynur; Atilla, Fatma Gül; Aksu, Güzide; Kütükçüler, Necil; Baylas, HalukSitokinler hücrelerin aktivasyonunu ve fonksiyonlarını yönlendirmeleri bakımından önemli konak faktörlerinin başında gelmektedir. Nonsteroid antienflamatuar ilaçlar siklooksijenaz enzimini inhibe ederek, kemik ve ekstraselüler matriks yıkımı ile sonuçlanan olaylar zincirini durdurabildikleri için çeşitli kronik enflamatuar hastalıkların tedavisinde kullanılmaktadır. Bu araştırmada, erişkin periodontitis tedavisinde mekanik tedaviye ek olarak verilen meloksikamının klinik periodontal ölçümler ve dişeti oluğu sıvısı sitokin seviyeleri üzerine etkileri araştırılmıştır. İncelenen klinik parametreler ve sitokin seviyeleri yönünden sadece mekanik tedavi yapılan grupta ve mekanik tedaviye ek olarak meloksikam verilen grupta 1.gün ve 10.gün değerleri arasında anlamlı farklar bulunmuştur. Gruplar arasındaki farklar istatistiksel olarak anlamlı değildir. Plasebo ve meloksikam gruplarının 10.gün IL-4, IF N-gama ve TGF-beta1 seviyeleri karşılaştırıldığında, birbirine çok yakın olduğu bulunmuştur (p>0.05). Onuncu gün değerleri yönünden, plasebo ve meloksikam gruplarının IL-6 seviyeleri arasında anlamlı fark olmamasına karşın, plasebo grubunda IL-6 seviyesinin 10.günde 1.güne göre arttığı, meloksikam grubunda ise tam tersine azaldığı saptanmıştır. Araştırmanın sonuçları, bir selektif COX-2 inhibitors olan meloksikamın PGE2 ve IL-6'yı inhibe etmesi nedeniyle periodontitis tedavisinde yararlı olabileceğini düşündürmektedir. Ancak, konunun aydınlatılabilmesi için daha ileri çalışmalara gereksinim vardır.Öğe Dietary supplementation of omega-3 fatty acid and circulating levels of interleukin-1 beta, osteocalcin, and C-reactive protein in rats(Amer Acad Periodontology, 2006) Vardar-Sengul, Saynur; Buduneli, Nurcan; Buduneli, Eralp; Kardesler, Levent; Baylas, Haluk; Atilla, Gul; Lappin, David; Kinane, Denis F.Background: In this study, we evaluated the effects of two different regimes of dietary supplementation of omega-3 fatty acid on serum levels of interleukin-1 beta (IL-1 beta), osteocalcin (OC), and C-reactive protein (CRP) in experimental periodontitis. Methods: Experimental periodontitis was induced by repeated injections of Escherichia coli lipopolysaccharide (LPS). Thirty-nine adult male Sprague-Dawley rats were divided into four study groups as follows: an LPS positive control group; a saline (negative) control group; and two different groups with omega-3 fatty acid dietary supplementation, one in which we gave the supplement subsequent to disease induction (TO3) and the other in which the agent was started prior to and continued subsequent to LPS injections (P + TO3). In the TO3 group, omega-3 fatty acid administration was performed for 14 days following induction of experimental periodontitis. In the P + TO3 group, omega-3 fatty acid was given for 14 days prior to the start of LPS injections and was continued for another 14 days subsequent to the induction of experimental periodontitis. On day 15 of the first LPS injection, serum samples were obtained and rats were sacrificed. Serum samples were analyzed for IL-1 beta, OC, and CRP concentrations by enzymelinked immunosorbent assay. Defleshed jaws were analyzed morphometrically for alveolar bone loss. Data were evaluated statistically by non-parametric tests. Results: LPS injection resulted in statistically significantly more bone loss compared to the saline control group (P < 0.05). None of the omega-3 fatty acid administration groups showed evidence that this fatty acid was effective in preventing LPS-induced alveolar bone loss. TO3 and P + TO3 groups revealed significantly higher IL-1 beta and OC levels than the LPS group (P < 0.05). The study groups exhibited no significant differences in the serum CRP levels. Conclusions: Omega-3 fatty acid administration does not seem to influence circulating levels of CRP. The significantly increased serum OC level observed in both omega-3 fatty acid regimes is curious and could have an effect on bone turnover, as could the further significant increase in serum IL-1 beta, which could counteract any osteoblastic induction by OC through promotion of osteoclast activity. The lack of a therapeutic benefit of omega-3 fatty acid in this study, despite the effects on OC and IL-1 beta, is difficult to explain, and further studies are required to more fully assess the potential role of this fatty acid in periodontal treatment.Öğe Effects of selective cyclooxygenase-2 inhibitor and omega-3 fatty acid on serum interleukin-1 beta, osteocalcin, and C-reactive protein levels in rats(Amer Acad Periodontology, 2006) Vardar-Sengul, Saynur; Buduneli, Nurcan; Buduneli, Eralp; Baylas, Haluk; Atilla, Gul; Lappin, David; Kinane, Denis F.Background: The aim of this study was to evaluate the effects of selective cyclooxygenase-2 inhibitor, celecoxib, and omega-3 fatty acid on serum levels of interleukin 1-beta (IL-1 beta), osteocalcin (OC), and C-reactive protein (CRP) in experimental periodontitis. Methods: Experimental periodontitis in rats was induced by repeated injection of purified lipopolysaccharide (LPS) derived from Escherichia coli endotoxin. Forty-seven adult male Sprague-Dawley rats were divided into five study groups as follows: saline control, LPS, LPS + celecoxib, LPS + omega-3 fatty acid, and LPS + celecoxib + omega-3 fatty acid. Celecoxib and omega-3 fatty acid were given alone or in combination during 14 days of the experimental study period. At the end of the 2-week protocol, serum samples were obtained, and the rats were sacrificed. Serum samples were analyzed for IL-1 beta, OC, and CRP concentrations by enzyme-linked immunosorbent assay. Defleshed jaws were analyzed morphometrically for alveolar bone loss. Data were evaluated statistically by non-parametric tests. Results: According to the morphometric measurements, the LPS and drug treatment groups showed significantly higher bone loss than the saline control group (P < 0.05). Omega-3 fatty acid, both alone and in combination with celecoxib, revealed significantly higher IL-1 beta levels than LPS and celecoxib groups (P < 0.05). Individual and combined administration of celecoxib and omega-3 fatty acid significantly increased OC levels compared to the LPS group (P < 0.05). There were no significant differences in serum CRP levels. Conclusions: Celecoxib and/or omega-3 fatty acid administration does not significantly influence circulating levels of CRP. The significantly increased serum OC level observed after individual and combination administration suggests that celecoxib and omega-3 fatty acid may in influence bone remodeling and thereby inhibit the progression of alveolar bone resorption. However, the failure to observe any significant inhibition of bone loss in celecoxib- and/or omega-3 fatty acid-treated rats compared to the LPS group suggests that their therapeutic effect may be reduced by other factors, such as increases in serum IL-1 beta promoting osteoclast activity.Öğe Expression Profile of Human Gingival Fibroblasts Induced by Interleukin-1 beta Reveals Central Role of Nuclear Factor-Kappa B Stabilizing Human Gingival Fibroblasts During Inflammation(Wiley, 2009) Vardar-Sengul, Saynur; Arora, Shilpi; Baylas, Haluk; Mercola, DanBackground: Interleukin (IL)-1 beta is a key cytokine in the pathogenesis of periodontitis, and it induces inflammatory mediators in periodontal diseases. We developed immortalized human gingival fibroblasts (HGFs), investigated the effects of IL-1 beta on the gene expression using expression arrays containing similar to 40,000 genes, and tested the role of nuclear factor-kappa B (NF-kappa B) in maintaining an activated HGF population. Methods: Total RNA was isolated from IL-1 beta-induced and mock-induced control cells. Gene expression analyses were performed using expression arrays and confirmed by quantitative real-time polymerase chain reaction. Western blot analysis to show inhibitor of kappa B-alpha (I kappa B alpha) phosphorylation and immunostaining of cells for NF-kappa B nuclear translocation were performed. Apoptosis was confirmed by assay of poly ADP-ribose polymerase (PARP) cleavage. Results: A total of 382 probe sets corresponding to 254 genes were differentially expressed in IL-1 beta-induced cells (P <0.001). A total of 215 genes were upregulated, and 39 genes were downregulated. Most notable NF-kappa B pathway members (NF kappa B1, NF kappa B2, I kappa B alpha, I kappa B epsilon, I kappa B zeta, REL, RELB, and TA-NFKBH) were upregulated. I kappa B alpha was phosphorylated, and NF-kappa B accumulated in the nucleus. An IL-1 beta-induced set of 27 genes was downregulated by an NF-kappa B inhibitor, leading to a decreased number of viable cells and suggesting an antiapoptotic role for NF-kappa B. Conclusions: IL-1 beta leads to a large number of significant expression changes consistent with a pathologic role in periodontitis, including enhancement of inflammatory cytokines, chemokines, transcription factors, matrix metalloproteinases, adhesion molecules, and especially NF-kappa B-dependent antiapoptotic genes. NF-kappa B activation blocks apoptosis, thereby stabilizing the HGF population in inflammation. J Periodontol 2009;80:833-849.Öğe Farklı tip periodontitis hastalarından alınan dişeti örneklerinde toplam proteoglikan ve kondroitin-4-sülfat seviyelerinin saptanması:(Ege Üniversitesi, 2003) Baylas, HalukDişeti, proteoglikanlar, kondroitin-4-sülfat, periodontitis.;Gingiva, proteoglycans, chondroitin-4-sulphate, periodontitis.Öğe Kronik ve agresif periodontisli bireylerde, IL-6, FC?RIIA VE FC?RIIIA gen polimorfizmi ile periodontal hastalığa duyarlılık arasındaki ilişkinin araştırılması(Ege Üniversitesi, 2006) Vardar Şengül, Saynur; Baylas, Haluk; Berdeli, Afig Hüseyinov; Han, BuketPeriodontitis, IL-6, FcGamma polymorphism, gingival crevicular fluid.;Periodontitis, IL-6, FcGamma, Polimorfizm, Dişeti Oluğu Sıvısı.Öğe Makrofaj migrasyonunu inhibe eden faktörün (MIF) -173 G/C polimorfizmi ile agresif ve kronik periodontitis arasındaki ilişkinin araştırılması(Ege Üniversitesi, 2008) Sayğan (Han), Buket; Baylas, HalukBu çalışmanın amacı şiddetli KP ve AgP hastaları ve periodontal olarak sağlıklı bireylerde MİF polimorfizminin incelenmesi, bu polimorfizmin; klinik periodontal parametreler, ve MİF'in dişeti oluğu sıvısındaki protein düzeyleri üzerine olan etkisinin araştırılmasıdır. 97 şiddetli KP, 123 şidddetli AgP ve 124 periodontal sağlıklı birey çalışmamıza dahil edildi. Periferik kan örneklerinden genomik DNA elde edildi. MİF - 173 G/C polimorfizmi PCR-RFLP yöntemi ile değerlendirildi. Alınan DOS örneklerinde, MİF protein seviyeleri ELİSA yöntemi ile değerlendirildi. Grupları arasında MİF -173 G/C polimorfizmi için genotip dağılımlar ve allel sıklıkları bakımından anlamlı bir fark saptanmadı. Lojistik regresyon analizinde bu polimorfizmin KP ve AgP için risk faktörü olmadığı belirlendi. DOS MİF protein düzeyleri başlangıçta AgP grubunda KP ve sağlıklı kontrol grubuna göre daha fazla bulundu. Çalışmamızda; Türk toplumunda MİF -173 G/C promoter polimorfizminin şiddetli KP ve AgP ile ilişkili olmadığını belirlenmiştir. Bu çalışma MİF' in periodontal hastalıklardaki etkisini araştıran ilk çalışmadır. Sonuçlarımıza göre şiddetli AgP grubunda DOS MİF protein düzeyleri sağlıklı gruba göre yüksek bulunmuştur. Bu noktadan hareketle daha geniş populasyonlar ile araştırmalar yapılmalıdır.;Polimorfizm, MİF, Agresif Periodontitis, Kronik Periodontitis, Dişeti oluğu sıvısı.;Polymorphism, MIF, aggressive periodontitis, chronic periodontitis, gingival crevicular fluid.Öğe Periodontal hastalık ile erken doğum ve düşük doğum ağırlığı arasındaki ilişkinin klinik, mikrobiyolojik ve biyokimyasal faktörler aracılığıyla değerlendirilmesi(Ege Üniversitesi, 2006) Baylas, Haluk; Buduneli, Nurcan[Abstract Not Available]Öğe Renin-angiotensin gene polymorphisms in relation to severe chronic periodontitis(Wiley-Blackwell Publishing, Inc, 2009) Gurkan, Ali; Emingil, Gulnur; Saygan, Buket Han; Atilla, Guel; Kose, Timur; Baylas, Haluk; Berdeli, AfigGurkan A, Emingil G, Saygan BH, Atilla G, Kose T, Baylas H, Berdeli A. Renin-angiotensin gene polymorphisms in relation to severe chronic periodontitis. J Clin Periodontol 2009; 36: 204-211. doi: 10.1111/j.1600-051X.2008.01379.x. Evidence suggests that the ultimate product of the renin-angiotensin system (RAS), angiotensin II, exerts inflammatory actions. The present study aimed to evaluate the inter-relation between gene polymorphisms of the RAS components; angiotensin converting enzyme (ACE), angiotensinogen (AGT) and angiotensin II type-I receptor (AT1R), and severe chronic periodontitis (CP). DNA was obtained from peripheral blood of 90 CP patients and 126 periodontally healthy subjects, and the clinical parameters were recorded. ACE I/D, AGT M235T and AT1R A1166C polymorphisms were genotyped by the PCR-RFLP method. Chi-square, anova and logistic regression methods were used in statistical analyses. The frequency of the ACE D allele was significantly lower in the CP group than the healthy group (p(corr)=0.015). CP subjects exhibited increased C allele carriage and C allele frequency of the AT1R gene (p(corr)=0.03 and p(corr)=0.03, respectively). All clinical parameters of CP patients were found to be similar in variant allele-carrying and non-carrying subjects (p > 0.05). The present findings suggest that ACE I/D and AT1R polymorphisms might be associated with susceptibility to CP but not with disease severity. The D allele of ACE I/D might be associated with decreased, whereas the C variant of AT1R A1166C might be associated with an elevated risk for CP in Turkish population.Öğe TLR2 Arg753Gly, TLR4 Asp299Gly and Thr399lle gene polymorphisms are not associated with chronic periodontitis in a Turkish population(Blackwell Publishing, 2007) Berdeli, Afig; Emingil, Guelnur; Saygan, Buket Han; Guerkan, Ali; Atilla, Guel; Koese, Timur; Baylas, HalukAim: Toll-like receptor (TLR) gene polymorphisms could affect the host's ability to respond to microbial pathogens. In this case-control study, the association of TLR2 and TLR4 gene polymorphisms with chronic periodontitis (CP) was investigated. Materials and Methods: Genomic DNA was obtained from the peripheral blood of 83 patients with CP and 106 periodontally healthy subjects. The TLR2 Arg753Gly, Arg677Trp and TLR4 Asp299Gly, Thr399Ile gene polymorphisms were genotyped by the polymerase chain reaction-restriction fragment length polymorphism method. The data were analysed by a chi(2) test, logistic regression analysis and the Mann-Whitney U test. Results: The 753Gln allele was found in 6.1% of the CP patients as compared with 6.6% in the control group (p > 0.05). The frequency of the 299Gly and 399Ile allele was 2.4% and 1.8% in CP patients. For the healthy subjects, the frequency was 2.8% for the 299Gly and 2.5% for the 399Ile allele (p > 0.05). None of the CP patients or healthy subjects showed homozygosity for the TLR2 and TLR4 alleles. Percentage of sites with bleeding on probing and plaque were significantly higher in 299Gly-positive patients compared with 299Gly-negative patients (p < 0.05). Conclusion: These results showed that the TLR2 and TLR4 gene polymorphisms studied are not associated with susceptibility to CP in Turkish patients.Öğe Toll-like receptor 2 and 4 gene polymorphisms in generalized aggressive periodontitis(Wiley, 2007) Emingil, Guelnur; Berdeli, Afig; Baylas, Haluk; Saygan, Buket Han; Guerkan, Ali; Koese, Timur; Atilla, GuelBackground: Toll-like receptors (TLRs) recognize exogenous ligands such as lipopolysaccharide and bacterial lipoprotein during the immune responses to pathogens. The aim of the present study was to investigate whether TLR2 and TLR4 gene polymorphisms are related to susceptibility to generalized aggressive periodontitis (GAgP). Methods: A total of 245 subjects were included in the present study. Genomic DNA was obtained from the peripheral blood of 90 patients with GAgP and 155 periodontally healthy subjects. Probing depth, clinical attachment loss, plaque accumulation, and bleeding on probing were recorded. The TLR2 gene Arg753Gln and Arg677Trp polymorphisms and TLR4 gene Asp299Gly and Thr3991le polymorphisms were genotyped by the polymerase chain reaction-restriction fragment length polymorphism method. The data were analyzed by chi(2) and Mann-Whitney U tests and logistic regression analysis. Results: There was no significant difference in the distribution of TLR2 and TLR4 genotypes and aliele frequencies between GAgP patients and healthy subjects (P > 0.05). The TLR2 753Gln allele was found in 3.9% of the GAgP patients compared to 6.1% in the healthy group. The GAgP patients and healthy subjects did not show homozygosity for the TLR2 mutant alleles. The TLR2 677Trp mutant allele was not found in any of the subjects; 2.2% of the GAgP patients and 2.9% of the periodontally healthy subjects were identified as having the TLR4 299Gly polymorphic allele. With regard to the TLR4 3991le polymorphic allele, 1.1% of the GAgP patients and 2.3% of the periodontally healthy subjects had this allele. Conclusions: The present study failed to find any significant association between the TLR polymorphisms and GAgP, potentially because of the small sample size. To the best of our knowledge, this was the first study to examine the prevalence of these polymorphisms in a Turkish population with aggressive periodontitis.