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Öğe Folic acid modified clay/polymer nanocomposites for selective cell adhesion(Royal Soc Chemistry, 2014) Barlas, F. B.; Seleci, D. Ag; Ozkan, M.; Demir, B.; Seleci, M.; Aydin, M.; Tasdelen, M. A.; Zareie, H. M.; Timur, S.; Ozcelik, S.; Yagci, Y.A folic acid (FA) modified poly(epsilon-caprolactone)/clay nanocomposite (PCL/MMT-(CH2CH2OH)(2)-FA) resulting in selective cell adhesion and proliferation was synthesized and characterized as a cell culture and biosensing platform. For this purpose, first the FA modified clay (MMT-(CH2CH2OH)(2)-FA) was prepared by treating the organo-modified clay, Cloisite 30B [MMT-(CH2CH2OH)(2)] with FA in chloroform at 60 degrees C. Subsequent ring opening polymerization of 3-caprolactone in the presence of tin octoate (Sn(Oct)(2)) using MMT-(CH2CH2OH)(2)-FA at 110 degrees C resulted in the formation of MMT-(CH2CH2OH)(2)-FA with an exfoliated clay structure. The structures of intermediates and the final nanocomposite were investigated in detail by FT-IR spectral analysis and DSC, TGA, XRD, SEM and AFM measurements. The combination of FA, PCL and clay provides a simple and versatile route to surfaces that allows controlled and selective cell adhesion and proliferation. FA receptor-positive HeLa and negative A549 cells were used to prove the selectivity of the modified surfaces. Both microscopy and electrochemical sensing techniques were applied to show the differences in cell adherence on the modified and pristine clay platforms. This approach is expected to be adapted into various bio-applications such as 'cell culture on chip', biosensors and design of tools for targeted diagnosis or therapy.Öğe Gold nanoparticle conjugated poly(p-phenylene-beta-cyclodextrin)-graft-poly(ethylene glycol) for theranostic applications(Wiley, 2019) Barlas, F. B.; Aydindogan, E.; Arslan, M.; Timur, S.; Yagci, Y.Fluorescent conjugated polymers gained interest in the last decades for both imaging and targeting tumor cells for the purpose of diagnosis and treatment of cancer. In the light of this objective conjugated poly(p-phenylene) possessing beta-cyclodextrin (beta-CD) units in the main chain and poly(ethylene glycol) side chains is used as an imaging and therapeutic agent to target U87 and Vero cells. Additionally, imaging quality and therapy efficiency of the bare graft copolymer and its gold nanoparticle (AuNP) conjugated form were investigated and compared. It is observed that beta-CD is effective not only for the imaging of the tumor cells, but also as a radiotherapy agent. Conjugation of the polymer with the AuNPs provides significant improvement in the therapeutic efficiency. (c) 2018 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019, 136, 47250.Öğe Gold nanoparticle loaded phytosomal systems: synthesis, characterization and in vitro investigations(Royal Soc Chemistry, 2014) Demir, B.; Barlas, F. B.; Guler, E.; Gumus, P. Z.; Can, M.; Yavuz, M.; Coskunol, H.; Timur, S.Most medicinal and pharmaceutical herbal extracts are poorly soluble in aqueous moieties and have reduced adsorption by living cells. Liposomal encapsulation of those so called phytosomes could be a solution to overcome this problem. Meanwhile, much research shows that metallic nanoparticles such as gold nanoparticles ( AuNPs) exhibit biological activity such as wound healing and antioxidant properties on living cells. Here, we constructed a novel liposomal formulation by encapsulating both Calendula officinalis extract and AuNPs. After the preparation of vesicles using the traditional thin film hydration method within extrusion, the resulting AuNP-phytosomes were characterized by dynamic light scattering size measurements, zeta potential and atomic force microscopy. These vesicles are less than 100 nm in size and have a high encapsulation efficiency for chlorogenic acid and quercetin as the model major molecules of Calendula extract. Furthermore, AuNP-phytosomes exhibited antioxidant and wound healing activity significantly according to the free forms of each encapsulated material and the plain liposome as well as the phytosome form. Moreover, the cellular interactions of the vesicles were monitored using the nano-vesicles prepared by Texas-Red labelled lipids under fluorescence microscopy.Öğe A multi-functional fluorescent scaffold as a multi-colour probe: design and application in targeted cell imaging(Royal Soc Chemistry, 2015) Kesik, M.; Demir, B.; Barlas, F. B.; Geyik, C.; Cevher, S. C.; Demirkol, D. Odaci; Timur, S.; Cirpan, A.; Toppare, L.A novel scaffold material based on a novel targeting strategy has been developed, benefiting from recent progress in the development of fluorescent bioprobes. This concept suggests that several specifications which are desired for cancer cell targeting and imaging studies can be satisfied at the same time in one multifunctional scaffold. Besides, such scaffolds exhibit multi-colour properties when combined with a targeting moiety. For this purpose, a fluorescent and functional monomer, 3-(1H-phenanthro[9,10-d] imidazol-2-yl) phenol (PIP) and an antibody labelling kit (CF555) were merged on the same scaffold to generate the proposed bioprobe. This design offers multicolour cell images by emitting at dual wavelengths with no quenching in its fluorescent property. Also, pendant alcohol groups in the structure of PIP enable covalent attachment to labelled protein, CF555/anti-CD44 in order to enhance the biological activity and specificity towards the target. After combining with the targeting moiety, the bioconjugate was characterized, tested for in vitro studies, and the cellular internalization was monitored in live cells via the fluorescence microscope technique. The present work with such a strategy explores the potential use of the proposed fluorescent probe for the first time. The aim is to achieve targeted imaging of CD44 positive U87-MG cancer cells and determine specific cellular labelling via fluorescence imaging and flow cytometry experiments.Öğe Radiolabeling of Phytohemagglutinin-L (L-PHA) Conjugated Magnetic Nanoparticles and Investigation In Vitro Biological Affinities(Springer, 2013) Unak, P.; Guldu, O. K.; Medine, F. E. I.; Kilcar, M. A. Y.; Barlas, F. B.; Muftuler, F. Z. B.; Timur, S.